iProof™ High-Fidelity DNA Polymerase, 20 U (172-5300) by Bio-Rad

Manufacturer Bio-Rad  |   Model: 1725300  |  Available Worldwide
20 U, 2 U/µl, stand-alone enzyme, includes 5x reaction buffers, MgCl2 solution, DMSO.


iProof™ High-Fidelity DNA Polymerase, 20 U (172-5300) by Bio-Rad product image
iProof™ High-Fidelity DNA Polymerase, 20 U (172-5300)


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iProof high–fidelity DNA polymerase from Bio–Rad Life Sciences comprises a unique Pyrococcus –like proofreading enzyme fused to a dsDNA binding protein, Sso7d.

This results in a thermostable polymerase that accurately amplifies long products from a variety of DNA templates.

iProof polymerase is available in three convenient formulations: a stand–alone enzyme, an easy–to–use master mix, and a PCR kit complete with controls.


All three formulations of Bio–Rad iProof high–fidelity DNA polymerase feature:

  • High fidelity — iProof DNA polymerase is 52–fold more accurate than Taq polymerase
  • Speed — high processivity dramatically reduces extension (15–30 sec/kb) and overall reaction times
  • Successful amplification of long products with higher yields — fragments up to 37 kb are amplified in less time and with less enzyme (0.25–1 unit/reaction)
  • Industry–Leading Fidelity — In a lacI –based fidelity assay, Bio–Rad iProof polymerase was shown to be more accurate than Taq , and a leading high–fidelity polymerase

Product Overview

iProof™ High-Fidelity DNA Polymerase, 20 U (172-5300) by Bio-Rad product image

iProof™ High-Fidelity DNA Polymerase, 20 U (172-5300)

Manufacturer Bio-Rad  |  Available Worldwide

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