Ion Total RNA-Seq Kit by Thermo Fisher Scientific Invitrogen

The Ion Total RNA-Seq Kit v2 includes the reagents needed to prepare representative cDNA libraries for strand-specific RNA sequencing on the Ion Personal Genome Machine (PGM™) sequencer. Either small RNA (such as microRNA) or whole transcriptome RNA samples can be prepared for next-generation sequencing. Version 2 of the Ion Total RNA-Seq Kit is an improvement over the first generation kit. The gel size selection step in the small RNA workflow has been replaced with a magnetic bead-based method. Magnetic bead-based purification replaces all of the filter cleanup steps and the total reaction time has been reduced to 6 hours.

The Ion Total RNA-Seq Kit v2 is designed to make cDNA library preparation for the PGM™ sequencer fast and flexible. It can be used to generate a representative cDNA library, flanked by specific sequences necessary for sequencing on the Ion PGM™ sequencer, from any type of RNA sample.

Designed as a complete solution with a common workflow, the Ion Total RNA-Seq Kit v2 combines optimized reagents and protocols for discovery of small RNAs and isoforms, coding RNA, noncoding RNA, and alternative splice variants.