The innuPREP Plasmid Small Kit provides a fast, simplified method of directly isolating plasmid DNA from a 25 µl bacterial culture. After performing an alkaline lysis step and precipitating chromosomal DNA and bacterial proteins, the next step is to bind plasmid DNA to a Spin Filter column. The need to pellet and then resuspend the bacteria is completely eliminated. The extraction process begins as soon as the bacterial culture is lysed; the process is complete in just 12 minutes. The resulting isolated plasmid DNA can then be used immediately in a variety of additional applications.