innuPREP Plasmid Mini Kit by Analytik Jena GmbH+Co. KG
Manufacturer Analytik Jena GmbH+Co. KG
innuPREP Plasmid Mini Kit
innuPREP Plasmid Mini Kit
Average Rating: 4.7
2 Scientists have reviewed this product
Status:
Reviewer
Member since: 2015
Organization: lmu
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- Ease of use
- After sales service
- Value for money
Great results
Rating: 5.0
Application Area: Plasmid isolation kit
"The kit is very easy to use with great results in terms of highly purified plasmid."
Review date: 15 Jan 2016 | innuPREP Plasmid Mini Kit
Status:
Reviewer Member since: 2013
Organization: University Of Pennsylvania
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- Ease of use
- After sales service
- Value for money
Rating: 4.3
Review date: 02 Dec 2013 | innuPREP Plasmid Mini Kit
Application Area:Purify Plasmid DNA
"This is a good alternative to the Qiagen kit but quicker and much more cost effective. I received the same level of yield and purity as I have with other competitors' products, and DNA worked well with an extra-chromosomal array."
Review date: 02 Dec 2013 | innuPREP Plasmid Mini Kit
The innuPREP Plasmid Mini Kit allows researchers to isolate plasmid DNA from bacterial lysates quickly and easily.
The method combines alkaline lysis with the process of binding plasmid DNA to a filter membrane once the chromosomal DNA and bacterial proteins have been precipitated. The bound plasmid DNA are then washed and eluted by adding a low salt buffer. The resulting isolated plasmid DNA can then be used immediately in a variety of additional downstream applications. The new method allows scientists to isolate plasmid DNA from 0.5–10 ml starting material. The use of a novel binding membrane makes it possible to isolate up to 40 µg of pDNA.
The method combines alkaline lysis with the process of binding plasmid DNA to a filter membrane once the chromosomal DNA and bacterial proteins have been precipitated. The bound plasmid DNA are then washed and eluted by adding a low salt buffer. The resulting isolated plasmid DNA can then be used immediately in a variety of additional downstream applications. The new method allows scientists to isolate plasmid DNA from 0.5–10 ml starting material. The use of a novel binding membrane makes it possible to isolate up to 40 µg of pDNA.
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