innuPREP Plasmid Mini Kit 2.0 by Analytik Jena GmbH+Co. KG

innuPREP Plasmid Mini Kit 2.0 allows the fast and easy isolation of plasmid DNA from bacteria cultures. To gain an optimal result protocol sequences, as well as the chemistry and used Spin Filters were adapted if compared to precursor products innuPREP Plasmid Mini Kit and innuPREP Plasmid Mini Kit Plus. The routine is based on the combination of an alkaline lysis and binding of the plasmid DNA to a filter membrane after precipitation of the chromosomal DNA and bacterial proteins. Bound plasmid DNA is then washed and finally eluted by using a low molar buffer. The isolated plasmid DNA can be used for a number of sensitive downstream applications, like transfection, cloning, PCR or sequencing directly. The new procedure also allows for processing of 0.5 ml to 15 ml of bacterial cultures based on the usage of a table centrifuge (after lysis). Due to the new filter membrane up to 80 µg pDNA can be isolated from one sample.