The innuPREP Blood DNA Midi Kit and the innuPREP Blood DNA Master Kit have been specially designed as an effective tool for isolating genomic DNA from 0.5 ml to 2 ml / 5 ml samples of whole blood.
The kits combine three steps: erythrocytes are first selectively digested, after which nucleated blood cells are pelleted and then lysed. After a precipitation step to selectively remove proteins, the DNA is bound to the surface of a Spin Filter membrane and then eluted. The extraction process involves the use of standard bench-top centrifuges and operates on a minicolumn scale. Combining a highly stringent Lysis Buffer with a novel Binding Buffer makes it possible to achieve unique yields of highly pure DNA.
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