The innuPREP Blood DNA Master Kit is used for extracting genomic DNA from 0.3 to 1 ml whole blood samples. The basis for the test is an optimum combination of three steps: selective digestion of erythrocytes, along with pelleting and subsequent lysing of nucleated blood cells. The lysis step is performed in what are known as PLP Tubes, which already contain all of the reagents required as well as the proteolytic enzymes, all in a stable form. This is followed by three additional steps: selective removal of proteins via precipitation, binding of the gDNA to a Spin Filter membrane, and the final elution. Standard bench-top centrifuges for mini-Spin Filters can be used despite the starting quantity in the master format.
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