The innuPREP Bacteria DNA Kit has been optimized specifically for isolating bacterial DNA from cell pellets after culturing, making it possible to process both gram+ and gram– bacteria.
The extraction process combines an initial lysis step using lysozyme and a subsequent proteolytic digestion step with a highly efficient process for binding bacterial DNA to the surface of a Spin Filter membrane. The DNA is then washed and desorbed from the surface of the filter. Extraction is based on Spin Filter columns and, in addition to being very easy to perform, also makes it possible to bind more than 50 µg of bacterial DNA. CE-IVD marking also makes the innuPREP Bacteria DNA Kit suitable for in-vitro diagnostics. Using the InnuPure® C16 extraction system in combination with the innuPREP Bacteria DNA Kit-IPC16 enables the automated extraction of bacterial DNA from up to 16 samples. The routine based on magnetic particle separation saves hands-on time and eases up nucleic acid extraction.