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Member since: 2014
Organization: University Hospital of Bonn
Application Area: Epigenetics, DNA Methylation, Free-Circulating DNA
"The innuCONVERT Bisulfite All-In-One kit is an easy-to-use product which allows for the bisulfite conversion of DNA from various sources. The protocol is very fast and enables a sample analysis including downstream analytics within one day. The performance of this kit is particularly high when applying formalin-fixed and paraffin-embedded tissues. In these cases the kit even works without preceding deparaffination. Therefore, tissues loss is minimized and the kit can also be applied to micro- and marcodissected tissues. The innuCONVERT Bisulfite Body Fluids kit is the only available kit which allows the extraction and bisulfite conversion of cell-free circulating DNA from high volumes (3ml) of body fluids (urine, blood serum and plasma). The protocol is quick and convenient. "
The innuCONVERT Bisulfite Purification Kit-IPC16 allows users to completely convert nonmethylated cytosine to uracil in just a few hours. DNA sample denaturation and bisulfite treatment are combined in the same reaction vessel. After a total reaction time of approx. 45 minutes, the converted DNA is isolated and desulfonated using the InnuPure® C16. It is possible to purify 16 samples in parallel. The eluted DNA is then ready-to-use in downstream applications (such as PCR, sequencing, etc.).
innuCONVERT Bisulfite Basic Kit
The innuCONVERT Bisulfite Basic Kit allows users to completely convert non-methylated cytosine to uracil in just a few hours. DNA sample denaturation and bisulfite treatment are combined in the same reaction vessel. After a total reaction time of approx. 3 hours, the converted DNA is isolated and desulfonated in a column. The eluted DNA is then ready-to-use in downstream applications (such as PCR, sequencing, etc.).
innuCONVERT Bisulfite All-In-One Kit
The innuCONVERT Bisulfite All-In-One Kit al-lows researchers to extract DNA from a variety of relevant starting materials and then completely convert non-methylated cytosine to uracil in a few hours. A specially optimized universal buffer can be used for processing different lysis protocols, while DNA sample/lysate denaturation and bisulfite treatment take place in a single reaction vessel. This is followed by on-column purification and desulfonation of the converted DNA. The pure nucleic acid obtained can then be easily used for later downstream applications (such as PCR, sequencing).
innuCONVERT Bisulfite Body Fluids Kit
The innuCONVERT Bisulfite Body Fluids Kit is a unique newcomer to the market for fast, accurate bisulfite conversion. This is the first kit that both isolates cell-free DNA from an exceptionally wide variety of body fluids and then completely converts non-methylated cytosine to uracil. A capacity for up to 3 mL serum, plasma, cell culture supernatants, ascites, pleural effusions, and other similar substances in conjunction with the ideal enrichment method (PME – Polymer Mediated Enrichment) yields top-of-the-line sensitivity. The entire process, including cell-free DNA extraction, a coupled denaturation/bisulfite conversion step, desulfonation and subsequent purification of the converted DNA (using a Spin Filter column), is complete in just a few hours. Direct downstream applications are available immediately.