CellTag™ 700 Stain In-Cell Western™ Kits
The CellTag™ 700 Stain ICW Kits provide detection reagents for cell-based In-Cell Western™ Assays.
Each kit includes blocking buffer, IRDye® 800CW secondary antibody for detection of a specific protein target in the 800 nm channel, and two fluorescent cell stains that are used in combination in the 700 nm channel to normalize well-to-well variations in cell number. This approach allows the target of interest to be detected accurately and cost-effectively.
CellTag 700 Stain is a near-infrared fluorescent, non-specific cell stain that provides accurate normalization to cell number for In-Cell Western applications. The stain accumulates in both the nucleus and cytoplasm of permeabilized cells, and provides linear fluorescent signal across a wide range of cell types and cell numbers. CellTag 700 Stain is applied to the cells during incubation with IRDye® 800CW secondary antibody, and enables accurate measurement of target protein levels with much higher throughput than Western blotting.