The In-Cell Western Kits provide detection reagents for cell-based In-Cell Western Assays.

Each kit includes blocking buffer, IRDye® 800CW secondary antibody for detection of a specific protein target in the 800 nm channel, and two fluorescent cell stains that are used in combination in the 700 nm channel to normalize well-to-well variations in cell number. This approach allows the target of interest to be detected accurately and cost-effectively.