Quickly test one or more kinases against dozens of peptides to identify potential substrates for subsequent IMAP screens.
The IMAP® Substrate Finder Kits are assay development tools that can accelerate the identification of substrates for kinases and be used to profile substrate specificity of a kinase. The IMAP Substrate Finder enables researchers to test kinases against a variety of peptides included in a 384-well microtiter plate to rapidly identify potential substrates for subsequent IMAP screens. Additionally, substrate specificity may be evaluated between kinase variants or among different mutants of the same kinase.
The Substrate Finder allows users to screen through dozens of predefined potential substrates for less than the cost of a few substrates. It saves time by testing kinases against more than 50 different peptides in a single 384-well plate. Once a substrate is identified, it can be used with IMAP in either FP or TR-FRET detection mode. Depending on the mode chosen, optimized conditions may vary.