Experience the benefit of multiplexed, cell- and bead-based assays performed during high content screening in a high throughput screening environment, ensuring heterogeneous responses can be captured earlier in the research and discovery of new therapeutics. Now multiplexed, homogeneous, bead and cell-based assays become easy to develop and run as robust primary screens. The cytometer can scan a plate in as little as 3 minutes, regardless of cell- or well-density while resolving objects at micron resolution in four colors simultaneously. These fast scan times and whole well analysis can gather data from >150,000 wells per 8-hour day, achieving the acquisition rates expected of high-throughput screening. The cytometer's speed comes from unique design elements including a large depth of focus and large field of view which eliminates the requirements to periodically focus or acquire multiple exposures to cover an entire field of view. A light scatter (labe free) mode allows generation of data from non-fluorescent objects (e.g., stem cell colonies where addition of dyes is unacceptable). To help simplify the workflow of moving suspension cytometry assays into the screening environment, the ImageXpress Velos Cytometer leverages a microplate-based environment to streamline evaluation of both adherent and suspension cell types, eliminating separation steps for adherent cells or colonies while eradicating daily routine procedures to prime and clean fluid lines. This well-based environment utilizes small aliquots (384- or 1536-well plates), preserving valuable stem or primary cells. Additionally, a microplate based assay allows samples to be rescanned to confirm results or gather additional content at a higher magnification on other systems.