Image Studio™ Lite - Free Western Blot Quantification Software by LI-COR Biosciences

"Great technical support. I got useful recommendations by email in less than 24 hours. I am still learning how to fully use the software, but so far it has been pretty easy to use and to learn. Quantification of bands for coomassie gel staining works well."

"Very easy to use an intuitive. Much better than other, more expensive software that I have used in the past. It was an easy transition to Image Studio Lite."

"The product helped me to quantify small amounts of tissue (areas from brains!) to understand the amounts of the protein that I'm working with. This is so useful!"

"High quality of image production and user-friendly software."

"Works great for western blots, which is all I’ve used it for."

"Very easy to use, clear methodology for background calculations, fast, saves your work easily. Since finding it, it is the only software I use."

"I've been using this software for the past 7-8 years and am extremely pleased with its ability to quantitate my western blot results."

"The programme is easy to use. Reproducible results. Support is readily available when needed."

"Product is user friendly. I use it mainly for western blot densitometry analysis. You can get your results quite quickly. Results are very reproducible and precise."

"It was very easy to use - even more so during the height of lockdown when the labs were shut down except for those needed for essential experiments. It made it a lot faster - and safer - for my colleagues who could send the blots to me to analyze, reducing their time in lab."

"Very good product, high quality, easy handling, very good service, very good results and software!"

"Effective instrument. Great for generating the data. I love its flexibility and user friendliness."

"I enjoy how you can not only use it with LI-COR instruments, but also images from 3rd parties. The "choose" function saves so much effort and the quantification is just fabulous. Also it is free!"

"Very user friendly software, especially useful in an environment where the turn over of people is high. Also compatible with other western imagers, very applicable. After sales care was okay."

"This free application allows you to analyze your LI-COR gel images at your desk. It is excellent for times when the instrument is used by a number of people and purchasing the full software package is too expensive. Our LI-COR instrument is great and they back it up with this good free analysis package for use at your desk. Customer service and support is outstanding."

"Great capabilities; requires learning curve to take advantage of all its abilities. I can easily get images, but doing the normalization and other items is really hard and takes a lot of non-intuitive clicking and modifying to get it to work."

"This is a very nice and free software from Li-Cor for quantitative analysis of western blots and dot blots etc. There is high feasibility to use this software even to quantify cell number and therefore I believe it will assist in studying scratch wound assays."

"Software was really helpful in all aspects, especially for measuring the intensity of western blot images, recommended for other colleagues as well."

"The software is pretty intuitive to use, which makes it quite easy. It helps me a lot to acquire robust data quickly, also considering the background (which is a bit of a problem when using imageJ, for instance). Adjustment of intensity allows for playing around with the signal to get the best quality without losing the pixel information."

"The software is really intuitive and easy to you. The results are also perfect and reproducible. "

"Awesome. Easy to use. Convenient and great support."

"I would definitely recommend this product. Image Studio Lite enabled me to extract and analyze data from almost every Western blot I conducted, even if the scan appeared useless at first. The different color channels and contrast settings were especially useful. One thing in the free version I would change is that the annotation of blot lanes was rather difficult. "

"Image Studio Lite works great for western analysis. I love that it has all the functionality of the full version."

"I would appreciate this software more if the plate/grid key had more customizable settings. We constantly find ourselves spending more time adjusting grids than actual analysis. We hope your company will continue to work on the plate/grid analysis feature for future upgrades. "

"This product is effective and easy to use. By using it, the process of analyzing the relative expression is simplified and time-saving."

"The one aspect I would change is the ability to edit shapes once placed on the image. It is very difficult to go back and select an image and adjust size. Sometime that feature works, most of the time it does not. I like the fact that the data can be easily exported to excel and being able to paste in sample names into the shape name while in the soft ware- makes tracking samples much easier when going back to an image weeks later."

"It is the best software that I have ever used. I think it is pretty useful. "

"The software is very user friendly and we've had a tremendous amount of support from our local LI-COR representative. The free version of the software is extremely valuable as it allows us to analyze data on multiple computers outside of the room that houses our LI-COR imaging equipment. I would highly recommend this software to anyone. "

"This software is SO user-friendly. You can teach yourself how to use it very quickly as it is set up very logically. I would highly recommend this to anyone!"

"The machine is really easy to use, and I really like the feature for quantifying the data right on the software. I would also like to say that the sensitivity is amazing, we only have to use a small amount of the antibody to image the westerns."

"I love the software. My only issue is that is does not have an updater built in and I have to re-download the software for updates "

"The software is free, so you can't beat the price."

"This is the best program for science data imaginable. "

"I consistently get great results with Image Studio. I use it almost daily for analysis of my western blots at my desk. It is a robust piece of software that enables quick, accurate, and reliable quantization of my western blots. I encourage all of my coworkers and labmates to use the software, it makes quantifying your proteins so simple. "

"Software is very effective and easy to use for scanning western blot membranes."

"I have both the Lite and Full version of this software so I can comment on the utility of the Lite version vs. Full. All of the basic and essential functions in the full version are in the lite version so I highly recommend it. If you want to use this for WB analysis, quantification, and saving your images in a very easy to use program and organization system then this is a solid choice. "

"Easy to use and reliable results comparing to ImageJ. The product sales representative stopped by the lab to give a training which was very useful. The only issue I have is when dealing with difficult antibodies, i.e., background is high, it shows 'inf' due to some spot on the band or if the band quality is not good enough, which is tolerable by human eyes."

"It is pretty easy to use and automates most of the analysis process. Also provides graphs and panels for publication format. "

"We have used Image Studio to do some RNA-EMSA experiments. It’s very user friendly with lots of options. Good thing is we can modify/enhance images after recording. "

"I do like Image Studio lite, however we have recently had some problems analyzing blots in which a few overexposed pixels were present in some bands. Image Studio lite refused to report density for these bands. When we took the images back to the LICOR machine software not only were the bands analyzed, no overexposed pixels were detected. Something seems off with this newer version because I hadn't had this problem with the old version."

"Very easy to use, the software immediately gives band intensity. I will definitely recommend to other users. "

"It is very easy to use. It helps me a lot in my research. I will definitely recommend it to my colleagues."

"It is very easy to use product. High sensitivity for Western blots and agarose gels. Easy to export data to other platforms."

"This software works great. I use it on my personal laptop and it allows me to visualize and quantify my blots outside of the lab."