iCycler iQ Real-Time PCR Detection System

Manufacturer Bio-Rad
4.0
/
5.0
  |  8 reviews


Product Image



Average Rating: 4.0
8 Scientists have reviewed this product

4 out of 5
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money
  • Status:

    Reviewer

  • Member since: 2011

  • Organization: UNIV. OF MIAMI-MILLER



  • Ease of use
    2 out of 5
    After sales service
    3 out of 5
    Value for money
    3 out of 5
Rating: 2.7
"Good overall machine, however it has a tough software interface which is not very simplistic."

Review date: 08 Jul 2011 | iCycler iQ Real-Time PCR Detection System
  • Status:

    Reviewer

  • Member since: 2011

  • Organization: AWU



  • Ease of use
    4 out of 5
    After sales service
    2 out of 5
    Value for money
    2 out of 5
Rating: 2.7
"This thermocycler has substantial well-position effects (see below). Bio-Rad, after wasting many hours trying to get this fixed eventually said that that these effects are within their normal specifications. I've also had trouble with the iCycler software. Using BioRad's EvaGreen control assay with all samples exactly the same and carefully pippeted using a multichannel pippetman into bio-rad clear non-skirted plates with bio-rad optical seals I get remarkably similar effects across two different runs. Specifically: -corner wells have a Ct value of ~0.25 higher -for every well more separating a well from the edge, the Ct value goes up ~0.035 (e.g. well A1 has a score of 0, A2=1, B2=2 and C6=7. This means that going from an edge well to the inner-most wells = 7*0.035 or 0.245 Ct value difference. Assuming a 100% efficiency to convert this Ct value to dilution factor (2^Ct=dilution factor) I have calculated that: This means the most inner wells will tend to have an ~19% lower means."

Review date: 17 May 2011 | iCycler iQ Real-Time PCR Detection System
  • Status:

    Reviewer

  • Member since: 2010

  • Organization: University Of Miami



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 5.0
"Gene expression assay for myosin after treatment."

Review date: 28 Jan 2011 | iCycler iQ Real-Time PCR Detection System
  • Status:

    Reviewer

  • Member since: 2009

  • Organization: IPK Gatersleben



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Rating: 4.3

Review date: 09 Nov 2009 | iCycler iQ Real-Time PCR Detection System


  • Status:

    Reviewer

  • Member since: 2008

  • Organization: UNIV. OF HONG KONG



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Rating: 4.0
"so far so good. a relatively inexpensive machine."

Review date: 04 Jul 2008 | iCycler iQ Real-Time PCR Detection System
  • Status:

    Reviewer

  • Member since: 2008

  • Organization: University of Miami, School of Medicine



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Rating: 4.3
"it is easy to use and gives very accurate results."

Review date: 03 Jul 2008 | iCycler iQ Real-Time PCR Detection System
  • Status:

    Reviewer

  • Member since: 2008

  • Organization: N.N. Petrov Institute of Oncology



  • Ease of use
    4 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
Rating: 3.7
"The system is easy to use and works non-stop reliably. Ours has yet to require maintenance."

Review date: 03 Jul 2008 | iCycler iQ Real-Time PCR Detection System
The iCycler iQ real-time PCR detection system is a sophisticated optical system that fits directly above the iCycler thermal cycler. It covers the widest range of excitation/emission wavelengths available, facilitating the greatest array of fluorescent PCR strategies. With this upgrade the iCycler thermal cycler expands the advantages PCR brings to your research.

Features:

  • Real-time analysis can be viewed online, during the PCR
  • Range of fluorophore excitation and emission from 400–700 nm
  • 4 different fluorophores can be multiplexed per sample tube
  • 96 samples can be tracked simultaneously, maximizing throughput
  • Sample data can be reanalyzed at any time
  • Raw data files are user accessible
  • Easy, intuitive software speeds setup and data presentation
  • Storage of hundreds of protocols
  • Standard PCR plate format accommodates existing protocols and minimizes costs
  • User interface offers easy yet powerful options for data analysis
  • Real-time quantitative PCR provides accurate quantitation, accelerating lead identification in drug discovery and improving viral load assessment
  • iCycler iQ software is designed to automate analysis options, including quantitative and melt-curve analysis; you can also choose to reanalyze raw data at any point post-run
  • Monitor the thermal protocol in real time, saving time and effort in your analysis
  • Closed-tube analysis reduces contamination concerns and speeds time to results


  • Practice of the patented polymerase chain reaction (PCR) process requires a license. The iCycler iQ system includes a licensed thermal cycler and may be used with PCR licenses available from Applied Biosystems. Its use with authorized reagents also provides a limited PCR license in accordance with the label rights accompanying such reagents. Some applications may require licenses from other parties.

    Product Overview

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    iCycler iQ Real-Time PCR Detection System

    Manufacturer Bio-Rad

    4.0 / 5.0 | 8 reviews