The iCE3 System performs free solution IEF in a capillary column (cIEF) and detects focused protein zones using a whole column UV absorption detector that avoids disturbing these focused zones. This technology is unique in that it has the comparable resolution of traditional gel IEF but incorporates the advantages of a column based separation technology, including quantitation and automation. It is superior to conventional cIEF performed with single point detection systems, which require a lengthy mobilization phase. As a result, method development and sample analysis can be run on the iCE3 in a fraction of the time required by other technologies and our resolution and reproducibility are superior. The iCE3, with whole-column detection, completely eliminates the need for a mobilization phase. This results in a unique combination of speed, resolution and reproducibility.