The HybriZAP-2.1 two-hybrid vector system, a eukaryotic system to detect proteinprotein interactions in vivo
, provides a method for the rapid identification of genes encoding proteins that interact with a bait protein. The system is based on the ability to separate eukaryotic transcriptional activators into two separate domains, the DNA binding domain (BD) and the transcriptional activation domain (AD).
- Detects protein-protein interactions in vivo using the yeast GAL4 two-hybrid system
- Uses a lambda vector for generating high-efficiency cDNA and genomic libraries with high percentage of inserts and increased representation
- Easily converts to plasmid format for library screening by in vivo mass excision3