Human IL-29 (IFN lambda 1) ELISA Ready-SET-Go!® 20 x 96 tests w/plates by eBioscience

IFN- lambdas are a novel family of interferons which mediate the induction of anti-viral protection in a wide variety of cells. The three members of the IFN- lambda family are lambda1, lambda2, and lambda3, also known as IL-29, IL-28A, and IL-28B, respectively. IFN- lambdas share with type I IFNs an intracellular signaling pathway that drives the expression of a common set of IFN-stimulated genes. IFN-lambdas induce multiple biological activities, including the upregulation of class I MHC gene product expression to levels comparable to those induced by IFN- alphas. IL-28 and IL-29 are tested for anti-viral activity by challenging the human hepatocellular carcinoma cell line HepG2 with infection by EMC (following pretreatment of the cells with cytokine). Consistent with a role in anti-viral protection, the mRNA expression of IFN-lambdas is detectable in cells infected with various viruses. Moreover, monocyte-derived dendritic cells (important producers of IFN- alpha) express IFN- lambda1 mRNA in response to treatment with dsRNA. TLR3 and TLR4 ligands induce IFN- alpha, IFN- beta, IL-28, and IL-29 gene expression in macrophages this is dependent upon IFN- alpha. IFN-lambdas mediate their anti-viral protection through a class II cytokine receptor complex distinct from that of type I IFNs. This is comprised of two essential receptor proteins, CRF2-12/IFN- lambdaR1, which is unique to IFN-lambdas, and CFR2-4/IL-10R2, which is shared with IL-10, IL-22, and IL-26 receptors. Whereas, the two chains of the type I IFN receptor (IFN-AR1 and IFN-AR2) and IL-10R2 are ubiquitously expressed, IFN- lambdaR1 expression is limited and cell-type dependent. IFN- lambdaR1 is not expressed by monocytes, but is up-regulated during GM-CSF/IL-4 induced differentiation of DCs from human monocytes, yielding iDCs which are fully responsive to IFN- lambda. The IFN- lambdas, IL-28 and IL-29, have recently been reported to prime dendritic cells to induce proliferation of Foxp3-bearing regulatory T cells. IFN- lambda-matured DCs express high levels of class I and II MHC gene products, but low levels of costimulatory molecules, and are able to specifically induce IL-2-dependent proliferation of CD4+CD25+FOXP3+ T cell population with contact dependent suppressive activity on T cells. This Human IL-29/IFN- lambda Ready-SET-Go! ELISA Set contains the necessary reagents, standards, buffers and diluents for performing quantitative enzyme-linked immunosorbent assays (ELISA). This ELISA set is specifically engineered for accurate and precise measurement of human IL-29 protein levels from samples including serum, plasma, and supernatants from cell cultures. The assay demonstrates parallelism in measuring recombinant and native human IL-29 proteins with a standard curve range of 8.0 pg/ml to 1,000 pg/ml, and assay sensitivity below 8.0 pg/ml. The assay has been validated by specific detection of significant levels (e.g., 500 pg/ml) of native human IL-29 protein in supernatants from human GM-CSF - derived dendritic cells activated for 24 hrs with polyIC and CD40-His.