HotStart DNA Polymerase

by Bioneer

5.0/5.0 (1 reviews)

Bioneer’s HotStart DNA polymerase uses an exclusive enzyme-mediated Hot Start PCR method that, unlike most other HotStart PCR chemistries, completely releases all polymerase activity during the first denaturation step. Top DNA polymerase is completely inhibited by pyrophosphate at temperatures below 70°C. However, at temperatures above 70°C, a thermostable pyrophosphatase initiates pyrophosphate hydrolysis and activates the DNA polymerase. This prevents the formation of non-specific products and primer-dimers during the reaction set-up process and results in improved PCR specificity.

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Average Rating: 5.0

Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
5 out of 5

Description

Bioneer’s HotStart DNA polymerase uses an exclusive enzyme-mediated Hot Start PCR method that, unlike most other HotStart PCR chemistries, completely releases all polymerase activity during the first denaturation step. Top DNA polymerase is completely inhibited by pyrophosphate at temperatures below 70°C. However, at temperatures above 70°C, a thermostable pyrophosphatase initiates pyrophosphate hydrolysis and activates the DNA polymerase. This prevents the formation of non-specific products and primer-dimers during the reaction set-up process and results in improved PCR specificity.

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HotStart DNA Polymerase

by Bioneer

5.0/5.0 (1 reviews)
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
5 out of 5






Receive your quote directly from Bioneer for HotStart DNA Polymerase

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