HitHunter™ Cortisol Assay by DiscoveRx Corporation
Manufacturer DiscoveRx Corporation
Cortisol is a steroid hormone that is produced and secreted by adrenal cortex. As the major glucocorticoid in the human body, it is involved in stress adaptation, blood pressure elevation and Na+ uptake, and has numerous effects on the immune system. The HitHunter™ Cortisol Assay Kit is an assay suitable for the measurement of cortisol in a competitive immunoassay format using EFC detection. Features and Benefits » Directly measure cortisol or cortisol expressed from microsomes » Flexible • Comp... Read more
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HitHunter™ Cortisol Assay
Cortisol is a steroid hormone that is produced and secreted by adrenal cortex. As the major glucocorticoid in the human body, it is involved in stress adaptation, blood pressure elevation and Na+ uptake, and has numerous effects on the immune system.
The HitHunter™ Cortisol Assay Kit is an assay suitable for the measurement of cortisol in a competitive immunoassay format using EFC detection.
Features and Benefits
» Directly measure cortisol or cortisol expressed from microsomes
» Flexible
• Compatible with cells or purified enzyme preparations
» Specific Antibody
• Does not bind to other molecules in the cortisol pathway
» Reduced Interference
• Chemiluminescent detection reduces interference from fluorescent compounds, lowering false positives
» Robust
• Large signal to background ratios for superior signal differentiation and assay performance
Measuring cortisol using HitHunter EFC Detection
HitHunter Cortisol Assay is a rapid, homogeneous method for measuring cortisol using Enzyme Fragment Complementation (EFC) as the detection method. Free cortisol competes for antibody binding against labeled ED-cortisol conjugate, a small peptide fragment of ß-galactosidase (ß-gal). Unbound ED-cortisol is free to complement with EA, an inactive ß-gal EFC enzyme, to form active enzyme, which subsequently hydrolyzes luminescent substrate for EFC detection by a microplate reader or CCD camera. The amount of signal generated is proportional to the amount of cortisol that is unbound by antibody.
Kit Contents
» ED-cortisol conjugate, EFC Label
» Cortisol Antibody
» EFC detection reagents
» Cortisol Standard
Instrument Requirements
» Luminometer, Multimode Microplate Reader or CCD Camera
Flexible Assay Characteristics
HitHunter™ Cortisol Assay is a reliable and simple method for the detection of cortisol. It is designed to be extremely flexible for a variety of formats suitable for discovery research for both therapeutic and HTS programs.
» Easily Automated Homogeneous protocols that are easily automated on existing liquid handlers
» Scaleable Adpatable protocols for 96-, 384-, and 1536-well applications
» Stable Signal Measurements can be made up to 24 hours
The HitHunter™ Cortisol Assay Kit is an assay suitable for the measurement of cortisol in a competitive immunoassay format using EFC detection.
Features and Benefits
» Directly measure cortisol or cortisol expressed from microsomes
» Flexible
• Compatible with cells or purified enzyme preparations
» Specific Antibody
• Does not bind to other molecules in the cortisol pathway
» Reduced Interference
• Chemiluminescent detection reduces interference from fluorescent compounds, lowering false positives
» Robust
• Large signal to background ratios for superior signal differentiation and assay performance
Measuring cortisol using HitHunter EFC Detection
HitHunter Cortisol Assay is a rapid, homogeneous method for measuring cortisol using Enzyme Fragment Complementation (EFC) as the detection method. Free cortisol competes for antibody binding against labeled ED-cortisol conjugate, a small peptide fragment of ß-galactosidase (ß-gal). Unbound ED-cortisol is free to complement with EA, an inactive ß-gal EFC enzyme, to form active enzyme, which subsequently hydrolyzes luminescent substrate for EFC detection by a microplate reader or CCD camera. The amount of signal generated is proportional to the amount of cortisol that is unbound by antibody.
Kit Contents
» ED-cortisol conjugate, EFC Label
» Cortisol Antibody
» EFC detection reagents
» Cortisol Standard
Instrument Requirements
» Luminometer, Multimode Microplate Reader or CCD Camera
Flexible Assay Characteristics
HitHunter™ Cortisol Assay is a reliable and simple method for the detection of cortisol. It is designed to be extremely flexible for a variety of formats suitable for discovery research for both therapeutic and HTS programs.
» Easily Automated Homogeneous protocols that are easily automated on existing liquid handlers
» Scaleable Adpatable protocols for 96-, 384-, and 1536-well applications
» Stable Signal Measurements can be made up to 24 hours
Product Overview
