HiSeq 2500 Sequencing System by Illumina

Manufacturer Illumina
4.5
/
5.0
  |  7 reviews


HiSeq 2500 Sequencing System by Illumina product image
HiSeq 2500 Sequencing System
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Average Rating: 4.5
7 Scientists have reviewed this product

4 out of 5
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money


Flexible, massive output, simple protocol, good after-sale service
Rating: 5.0

  • Application Area: Whole genome and targeted DNA sequencing

"Very simple and direct protocol, from checking the quality of DNA to running the sequencing libraries in HiSeq. Most importantly, HiSeq offers such flexibility, I can sequence organisms with smaller genome to organisms with huge genome. What I need is to know how much output I need, then I can select Single Flow Cell or Double Flow Cell, high output mode or rapid run mode, then select the read length I prefer, ranging from 1x36bp to 2x125bp. I can even customize the read length I prefer. There is even an online Illumina Sequencing Coverage Calculator. With this calculator, I know what mode to go for, what kit to buy and how deep the coverage of the sample. The output for the HiSeq 2500 is huge. Thus, it has a relatively low cost-per-base. Illumina has this BaseSpace apps, in which you can install into your phone and monitor your sequencing experiment. You can even log into BaseSpace via your computer to monitor your run and even share your experiment monitoring with your colleagues. The after-sale service is good, as they will try they very best to answer my questions. When my sequencing experiments failed due to reagents or instrument issue, the company will replace the kits."

Review date: 23 Jan 2017 | HiSeq 2500 Sequencing System
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: RCAST, The University of Tokyo



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Instrument needed!
Rating: 4.0

  • Application Area:Genomics and epigenomics work

"Our research would be very difficult or maybe even impossible without use of an illumina sequencer."

Review date: 17 Jan 2017 | HiSeq 2500 Sequencing System
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: MedGenome labs



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Better Chemistry, service and easy use. What else do we require?
Rating: 5.0

  • Application Area:DNA sequencing

"Yes, it is very easy to use and better Chemistry and the service is good so I recommend this product."

Review date: 28 Jul 2016 | HiSeq 2500 Sequencing System
  • Status:

    Advanced Reviewer

  • Member since: 2013

  • Organization: Beth Israel Deaconess Medical Center



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great Results! Love it!
Rating: 5.0

  • Application Area:To analyze Whole exome sequencing data

"The results are really fantastic and the technical support provided is top class"

Review date: 29 Jun 2016 | HiSeq 2500 Sequencing System
  • Status:

    Advanced Reviewer

  • Member since: 2013

  • Organization: Beth Israel Deaconess Medical Center



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Great sensitivity
Rating: 4.3

  • Application Area:Sequencing variants

"It is easy to use and reproducible for genetic testing"

Review date: 24 Nov 2015 | HiSeq 2500 Sequencing System
  • Status:

    Reviewer

  • Member since: 2006

  • Organization: Rutgers, The State University of New Jersey



  • Ease of use
    3 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Rating: 3.7

  • Application Area:Human Genetics

"The 2500 is complicated and requires significant computer support. However, once it gets started it’s a workhorse and it functions continuously. "

Review date: 22 Apr 2014 | HiSeq 2500 Sequencing System
  • Status:

    Reviewer

  • Member since: 2008

  • Organization: NIBMG



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Rating: 4.7

  • Application Area:Genomics

"This is the best Next Generation Sequencing platform on the market. "

Review date: 09 Apr 2014 | HiSeq 2500 Sequencing System
Power and efficiency for large-scale genomics.

The HiSeq 2500 System features two run modes, rapid run and high output run mode, and the ability to process one or two flow cells simultaneously. This provides a flexible and scalable platform that supports the broadest range of applications and study sizes. No other system lets you perform so many applications so quickly and easily. Choose between rapid run and high output modes offering scalable output to fit your project needs. New reagents enable the generation of up to 1 terabase (Tb) of data in high output mode, supporting the greatest number of samples per run.

Rapid run mode provides quick results, allows efficient processing of a limited number of samples, and offers support of longer paired-end 150 base pair reads, which enable greater depth of coverage and improved assembly for de novo applications.

High output mode is ideal for larger studies or when the greatest depth of coverage is required. High output mode allows you to batch process > 6× more samples than rapid mode and to complete large projects quickly and efficiently. New HiSeq SBS Kits v4 produce 4 billion clusters per run, generating up to 1 Tb of data in 6 days.