The HIS-Select iLAP (Integrated Lysis and Affinity Purification) Columns combine cell lysis and protein purification steps into one. These single-use, disposable columns are designed for the one-step purification of histidine-tagged proteins directly from a 5 mL bacterial culture. The single-step method uses Sigma's patented iLAP technology, which allows quick and simple histidine-tagged protein purification from recombinant clones. Each column contains nickel chelate resin for the purification, as well as five lysis reagent tablets, which include all the necessary detergents and enzymes needed for efficient cell lysis and protein extraction while eliminating the need to harvest the cells from culture. Each column is capable of purifying at least 1 mg of histidine-tagged protein directly ...Read more
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