His GraviTrap TALON® is a prepacked gravity flow column for convenient manual purification of histidine-tagged proteins. Ready-to-use gravity flow column prepacked with 1 ml TALON® Superflow™ cobalt-IMAC (immobilized metal affinity chromatography) medium.
His GraviTrap TALON® columns are delivered in a package that can be converted into a column stand to simplify purification, eliminating the need for additional equipment to complete the protocol. Special column frits protect the chromatography medium from running dry during purification.
His GraviTrap TALON Features:
•Convenient histidine-tagged purification with high purity (> 90%) and binding capacity of up to 15 mg/column.
•Ready-to-use gravity flow column prepacked with 1 ml TALON® Superflow™ cobalt-IMAC (immobilized metal affinity chromatography) medium offering a different selectivity compared to nickel-charged media.
•One run takes about 30 minutes, and large sample volumes can be applied all at once.