Status:
Advanced ReviewerMember since: 2014
Organization: Pad.Dr.D.Y.Patil Institute of pharmaceutical sciences and research
Excellent , easy to use ,wonderful equipment
Application Area: for pharmaceutical purpose
"HILIC is certainly becoming an established technique for the analysis of polar and or basic substances. In comparison to reversed phase many advantages have been highlighted. This technique should be evaluated where appropriate as several key features allow for faster and more efficient analyses to be carried out. The low viscosity of the eluents utilised with HILIC are highly desirable especially in terms of analytical throughput. Such low viscosities are only achievable with reversed-phase systems by means of applying elevated temperatures. Furthermore, the application of elevated temperature in RP-LC is mainly restricted by the availability of thermally stable stationary phases. Of the few disadvantages, the reliance on acetonitrile only seems to be of concern during times of shortage. Solubility issues may certainly be an ongoing challenge with some analytes. However, this slight inflexibility can be addressed using fine adjustment of the injection diluent and mobile phase composition. "