Use the High Pure FFPE RNA Micro Kit to isolate total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue samples for direct use in RT-PCR. The isolated RNA is suitable for relative quantification of mRNA in RT-PCR using real-time PCR systems such as the LightCycler^® Carousel-Based System and the LightCycler^® 480 System.

Isolate total RNA from 1 to 10 µm sections of formalin-fixed, paraffin-embedded tissue (e.g., colon, breast, liver, kidney, or spleen of mammalian species, including human research samples). Section thickness, tissue type, age of sample, and the fixation protocol used can affect yield and quality of the isolated RNA.
 
Benefits 

• Save time with a simple and rapid protocol.
• Obtain a highly concentrated (10 µl) eluate and recovery (>80%).
• Isolate DNA-free RNA, ideal for use in qualitative and relative quantitative RT-PCR.
• Efficiently isolate total RNA – even small RNA fragments.
• Generate high-quality template RNA that shows excellent performance and linearity in RT-PCR.
• Rely on the innovative column design to efficiently recover contamination-free RNA.