The DRG Hepcidin-25 (bioactive) ELISA is an enzyme immunoassay for the quantitative measurement of Hepcidin-25 in serum and plasma. (For research use only) The DRG Hepcidin-25 ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA), based on the principle of competitive binding. The microtiter wells are coated with a monoclonal (mouse) antibody directed towards an antigenic site of the Hepcidin-25 molecule. Endogenous Hepcidin-25 of a specimen sample competes with a Hepcidin-25-biotin conjugate for binding to the coated antibody. After incubation, the unbound conjugate is washed off and a streptavidin-peroxidase enzyme complex is added to each well. After incubation, unbound enzyme complex is washed off and substrate solution is added. The blue colour development is stopped after a short incubation time, turning the colour from blue to yellow. The intensity of colour developed is reverse proportional to the concentration of Hepcidin in the specimen sample. (U.S. Patents 7,320,894, 7,411,048 and 7,998,691)
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