Hepcidin-25 (bioactive) ELISA Kit EIA-5258

4.0
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5.0
  |  7 reviews


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Average Rating: 4.0
7 Scientists have reviewed this product

4 out of 5
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money


Rating: 3.7

  • Application Area: analyze Hepcidin-25 levels in plasma samples

"The DRG International Hepcidin-25 ELISA is a very simple and reliable assay. I have run multiple plasma samples. The total time from set up to data collection is 3 hours incubation 1.75 hours incubation. The only negative aspect of the kit is the price. However, this is the only Hepcidin-25 ELISA kit available for in-lab use. All other companies require you to send your samples to them for analysis."

Review date: 07 Aug 2015 | Hepcidin-25 (bioactive) ELISA Kit EIA-5258
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: clinilab



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    3 out of 5
Effective in anemia and hemochromatosis. The work is easy with a short incubation period, giving quantitative results.
Rating: 4.0

  • Application Area:serum application

"Effective in anemia and hemochromatosis. The work is easy with a short incubation period, giving quantitative results. "

Review date: 31 Jul 2015 | Hepcidin-25 (bioactive) ELISA Kit EIA-5258
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: synlab LMZ



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 5.0

  • Application Area:for routine and clinical trials samples

"Easy to use, CE labelled, 2 controls, short incubation time,"

Review date: 30 Jan 2015 | Hepcidin-25 (bioactive) ELISA Kit EIA-5258
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: Palex Medical



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Rating: 4.3

  • Application Area:For iron homeostasis Studies

"We obtained very good results. Serum ferritin and HFE genotyping. Serum samples was measured at the Hospital Clinic Barcelona central laboratory using a commercially available automated ELISA assay"

Review date: 30 Jan 2015 | Hepcidin-25 (bioactive) ELISA Kit EIA-5258
  • Status:

    Reviewer

  • Member since: 2014

  • Organization: Hershey Medical Center PSU



  • Ease of use
    3 out of 5
    After sales service
    2 out of 5
    Value for money
    1 out of 5
Rating: 2.0
"This product was not consistent with results and standards. There was some tech support but sometimes it wasn't helpful."

Review date: 01 Sep 2014 | Hepcidin-25 (bioactive) ELISA Kit EIA-5258


  • Status:

    Advanced Reviewer

  • Member since: 2007

  • Organization: Pfizer Inc



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 5.0

  • Application Area:pharmaceutical safety assessment

"This kit has been a joy to work with, the effort put into the creation of this assay is impressive. We have validated its use for both humans and non-human primates, and we get very good results in rats. The technical service and company support during our validation was invaluable."

Review date: 01 Sep 2014 | Hepcidin-25 (bioactive) ELISA Kit EIA-5258
  • Status:

    Reviewer

  • Member since: 2014

  • Organization: Rhode Island Hospital



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Rating: 4.3

  • Application Area:medical research

"DRG kit is the only available one for serum hepcidin detection. The assay itself is quite reliable. The tech support is very helpful! They not only try to help realtime but also follow-up well."

Review date: 01 Sep 2014 | Hepcidin-25 (bioactive) ELISA Kit EIA-5258
The DRG Hepcidin-25 (bioactive) ELISA is an enzyme immunoassay for the quantitative  measurement of Hepcidin-25 in serum and plasma. (For research use only)

The DRG Hepcidin-25 ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA), based on the principle of competitive binding. The microtiter wells are coated with a monoclonal (mouse) antibody directed towards an antigenic site of the Hepcidin-25 molecule. Endogenous Hepcidin-25 of a specimen sample competes with a Hepcidin-25-biotin conjugate for binding to the coated antibody.

After incubation, the unbound conjugate is washed off and a streptavidin-peroxidase enzyme complex is added to each well. After incubation, unbound enzyme complex is washed off and substrate solution is added. The blue colour development is stopped after a short incubation time, turning the colour from blue to yellow. The intensity of colour developed is reverse proportional to the concentration of Hepcidin in the specimen sample.