Hepcidin-25 (bioactive) ELISA Kit EIA-5258

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Hepcidin-25 (bioactive) ELISA Kit EIA-5258
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The DRG Hepcidin-25 (bioactive) ELISA is an enzyme immunoassay for the quantitative  measurement of Hepcidin-25 in serum and plasma. (For research use only) The DRG Hepcidin-25 ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA), based on the principle of competitive binding. The microtiter wells are coated with a monoclonal (mouse) antibody directed towards an antigenic site of the Hepcidin-25 molecule. Endogenous Hepcidin-25 of a specimen sample competes with a Hepcidin-25-biotin conjugate for binding to the coated antibody. After incubation, the unbound conjugate is washed off and a streptavidin-peroxidase enzyme complex is added to each well. After incubation, unbound enzyme complex is washed off and substrate solution is added. The blue colour development is stopped...Read more

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Reviews

5 Scientists have reviewed this product


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Average Rating: 4.1

Ease of use
4 out of 5
After sales service
4 out of 5
Value for money
4 out of 5
Rating: 4.3
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money
4 out of 5
  • Review date:30 Jan 2015

  • Application Area:For iron homeostasis Studies

  • Hepcidin-25 (bioactive) ELISA Kit EIA-5258

"We obtained very good results. Serum ferritin and HFE genotyping. Serum samples was measured at the Hospital Clinic Barcelona central laboratory using a commercially available automated ELISA assay"
Rating: 2.0
Ease of use
3 out of 5
After sales service
2 out of 5
Value for money
1 out of 5
  • Review date:01 Sep 2014

  • Hepcidin-25 (bioactive) ELISA Kit EIA-5258

"This product was not consistent with results and standards. There was some tech support but sometimes it wasn't helpful."
Rating: 5.0
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
5 out of 5
  • Review date:01 Sep 2014

  • Application Area:pharmaceutical safety assessment

  • Hepcidin-25 (bioactive) ELISA Kit EIA-5258

"This kit has been a joy to work with, the effort put into the creation of this assay is impressive. We have validated its use for both humans and non-human primates, and we get very good results in rats. The technical service and company support during our validation was invaluable."
Rating: 4.3
Ease of use
4 out of 5
After sales service
5 out of 5
Value for money
4 out of 5
  • Review date:01 Sep 2014

  • Application Area:medical research

  • Hepcidin-25 (bioactive) ELISA Kit EIA-5258

"DRG kit is the only available one for serum hepcidin detection. The assay itself is quite reliable. The tech support is very helpful! They not only try to help realtime but also follow-up well."

Description

The DRG Hepcidin-25 (bioactive) ELISA is an enzyme immunoassay for the quantitative  measurement of Hepcidin-25 in serum and plasma. (For research use only)

The DRG Hepcidin-25 ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA), based on the principle of competitive binding. The microtiter wells are coated with a monoclonal (mouse) antibody directed towards an antigenic site of the Hepcidin-25 molecule. Endogenous Hepcidin-25 of a specimen sample competes with a Hepcidin-25-biotin conjugate for binding to the coated antibody.

After incubation, the unbound conjugate is washed off and a streptavidin-peroxidase enzyme complex is added to each well. After incubation, unbound enzyme complex is washed off and substrate solution is added. The blue colour development is stopped after a short incubation time, turning the colour from blue to yellow. The intensity of colour developed is reverse proportional to the concentration of Hepcidin in the specimen sample.

Product Overview

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Hepcidin-25 (bioactive) ELISA Kit EIA-5258

by DRG International Inc.

4.1/5.0 (5 reviews)
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money
4 out of 5






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