Hepcidin 25 (bioactive) by DRG International Inc.

Manufacturer DRG International Inc.  |   Model: HYE-5769  |  Available Worldwide
4.0
/
5.0
  |  7 reviews
High Quality Assays with Reproducible and Reliable Results


Hepcidin 25 (bioactive) by DRG International Inc. product image
Hepcidin 25 (bioactive)
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Average Rating: 4.0
7 Scientists have reviewed this product

4 out of 5
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money


Rating: 3.7

  • Application Area: analyze Hepcidin-25 levels in plasma samples

"The DRG International Hepcidin-25 ELISA is a very simple and reliable assay. I have run multiple plasma samples. The total time from set up to data collection is 3 hours incubation 1.75 hours incubation. The only negative aspect of the kit is the price. However, this is the only Hepcidin-25 ELISA kit available for in-lab use. All other companies require you to send your samples to them for analysis."

Review date: 07 Aug 2015 | Hepcidin 25 (bioactive)
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: clinilab



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    3 out of 5
Effective in anemia and hemochromatosis. The work is easy with a short incubation period, giving quantitative results.
Rating: 4.0

  • Application Area:serum application

"Effective in anemia and hemochromatosis. The work is easy with a short incubation period, giving quantitative results. "

Review date: 31 Jul 2015 | Hepcidin 25 (bioactive)
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: synlab LMZ



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 5.0

  • Application Area:for routine and clinical trials samples

"Easy to use, CE labelled, 2 controls, short incubation time,"

Review date: 30 Jan 2015 | Hepcidin 25 (bioactive)
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: Palex Medical



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Rating: 4.3

  • Application Area:For iron homeostasis Studies

"We obtained very good results. Serum ferritin and HFE genotyping. Serum samples was measured at the Hospital Clinic Barcelona central laboratory using a commercially available automated ELISA assay"

Review date: 30 Jan 2015 | Hepcidin 25 (bioactive)
  • Status:

    Reviewer

  • Member since: 2014

  • Organization: Hershey Medical Center PSU



  • Ease of use
    3 out of 5
    After sales service
    2 out of 5
    Value for money
    1 out of 5
Rating: 2.0
"This product was not consistent with results and standards. There was some tech support but sometimes it wasn't helpful."

Review date: 01 Sep 2014 | Hepcidin 25 (bioactive)
  • Status:

    Advanced Reviewer

  • Member since: 2007

  • Organization: Pfizer Inc



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 5.0

  • Application Area:pharmaceutical safety assessment

"This kit has been a joy to work with, the effort put into the creation of this assay is impressive. We have validated its use for both humans and non-human primates, and we get very good results in rats. The technical service and company support during our validation was invaluable."

Review date: 01 Sep 2014 | Hepcidin 25 (bioactive)
  • Status:

    Reviewer

  • Member since: 2014

  • Organization: Rhode Island Hospital



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Rating: 4.3

  • Application Area:medical research

"DRG kit is the only available one for serum hepcidin detection. The assay itself is quite reliable. The tech support is very helpful! They not only try to help realtime but also follow-up well."

Review date: 01 Sep 2014 | Hepcidin 25 (bioactive)
The DRG:HYBRiD-XL Hepcidin 25 (bioactive) is an enzyme immunoassay for the quantitative in vitro diagnostic measurement of Hepcidin 25 in serum or plasma (EDTA-, heparin-or citrate plasma).Only for use with the DRG:HYBRiD-XL Analyzer.Hepcidin is an iron homoeostasis regulator peptide. The bioactive peptide Hepcidin 25 is generated predominantly in the liver by proteolytic cleavage of the C-terminal 25 amino acids of prohepcidin (1). Subsequent N-terminal processing of Hepcidin 25 results in smaller peptides of 20-24 amino acids that show greatly reduced activity and accumulate in the urine (2). Although originally identified as antimicrobial peptide (3), Hepcidin 25 is now established as a major regulator of dietary iron absorption and cellular iron release (4). Hepcidin exerts its regulatory function by counteracting the function of ferroportin, the major cellular iron exporter in the membrane of macrophages, hepatocytes, and the basolateral site of enterocytes. Hepcidin 25 induces the internalization and degradation of ferroportin, resulting in increased intracellular iron stores, decreased dietary iron absorption, and decreased circulating iron concentrations (5). Hepatocellular hepcidin synthesis decreases under conditions of increased demand for circulating iron like iron deficiency, hypoxia, anemia, and erythropoiesis. In contrast, hepcidin synthesis is induced by inflammation and infection (6). Serum Hepcidin 25 has been shown to add value to identify and differentiate specific disease conditions. Hepcidin deficiency causes hereditary hemochromatosis, characterized by body iron overload that may progress to liver cirrhosis (7). In addition, low Hepcidin 25 concentration can be induced by iron loading anemias (8) and chronic hepatitis C (9).  In contrast, high Hepcidin 25 levels have been found in iron-refractory iron-deficiency anemia (10), during infection, chronic kidney disease (11), and after intensive exercise, explaining the high iron deficiency among athletes (12).The DRG:HYBRiD-XL Hepcidin 25 (bioactive) Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the principle of competitive binding. The antibody coated wells (ACW) of the reagent cartridges are coated with a monoclonal [mouse] antibody directed towards a unique antigenic site of the Hepcidin 25 molecule. Endogenous Hepcidin 25 of a patient sample competes with a Hepcidin 25-Biotin conjugate (Enzyme Conjugate) for binding to the coated antibody. After incubation the unbound conjugate is washed off. Bound Conjugate is detected by Streptavidin-HRP (Enzyme Complex). After incubation the unbound complex is washed off. The amount of bound enzyme complex is inversely proportional to the concentration of Hepcidin 25 in the sample. Having added the substrate solution, the intensity of colour developed is inversely proportional to the concentration of Hepcidin 25 in the patient sample.

Product Overview

Hepcidin 25 (bioactive) by DRG International Inc. product image

Hepcidin 25 (bioactive)

Manufacturer DRG International Inc.  |  Available Worldwide

4.0 / 5.0 | 7 reviews