HEK293 AequoScreen® Parental cell line by Revvity

Manufacturer Revvity
AequoScreen® Parental cells stably expressing the mitochondrially targeted apoaequorin for flash-luminescence assay. Recombinant, in HEK293 host cell. GPCRs of your choice can be transiently / stably transfected into these cell lines. Following GPCR stimulation, increases in intracellular calcium enable measurement of the resulting flash luminescence signal. Two vials of cryopreserved cells are shipped per order. Feature/Benefits: Functional, homogenous and generic assay platform for Gq co... Read more
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HEK293 AequoScreen® Parental cell line by Revvity product image
HEK293 AequoScreen® Parental cell line

Revvity



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AequoScreen® Parental cells stably expressing the mitochondrially targeted apoaequorin for flash-luminescence assay.
Recombinant, in HEK293 host cell. GPCRs of your choice can be transiently / stably transfected into these cell lines. Following GPCR stimulation, increases in intracellular calcium enable measurement of the resulting flash luminescence signal. Two vials of cryopreserved cells are shipped per order.

Feature/Benefits:

  • Functional, homogenous and generic assay platform for Gq coupled GPCRs and other calcium signalling proteins.
  • Fully automatable and miniaturizable to 1536
  • Enables reduction in reagents and consumable costs
  • High signal to background ratio enabling detection of agonists, antagonists and allosteric modulators
  • Robust assay (Z, CV values, solvent resistance)
  • Reduces the number of false positives from autofluorescent compounds in screening libraries
  • Option to work with cells in suspension to increase throughput

Product Overview

HEK293 AequoScreen® Parental cell line by Revvity product image

HEK293 AequoScreen® Parental cell line

Manufacturer Revvity

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