Taking the lead in PTM Discovery
The HCTultra PTM Discovery System™ features ETD: Electron Transfer Dissociation, a fragmentation technique that preserves post-translational modifications
Modern functional proteomics demands a step beyond protein identification – it is becoming more and more essential to unravel the many modifications proteins undergo triggering their biological activity.
Unique capabilities for PTM discovery
With ETD, scientists are enabled for specific information-rich PTM (post-translational modifications) analysis on the unsurpassed HCTultra PTM Discovery System™. ETD allows peptide and protein fragmentation while preserving modifications such as phosphorylation or glycosylation. This gives the researcher easy access to protein sequencing and simultaneous identification of type and location of various PTMs.
Powerful de novo sequencing
ETD MS/MS spectra of peptides can now be collected on-the-fly during LC/MS/MS runs using the HCTultra PTM Discovery System. Due to its non-ergodic nature, ETD typically creates very clean MS/MS spectra with intact PTMs. On the HCTultra PTM Discovery System, the new ETD implementation often provides complete amino acid series, without the low-mass cut-off traditionally encountered in ion trap MS/MS. The combination with the superior mass accuracy of the HCTultra enables powerful de novo sequencing capabilities. Using the PhosphoScan™ mode of the HCTultra with ETD offers a unique and powerful system for single run auto-LC/MS/MS/MS CID/ETD experiments specifically for phosphorylation analysis.