The separation of carbohydrates by anion exchange at basic pH is possible because each carbohydrate carries a different negative charge at basic pH. To utilize the full potential of the RCX-10 column (gradient separations) a Pulsed Amperometric Detector (PAD) is recommended. A PAD allows utilization of both gradient and isocratic elution for the separation of carbohydrates. A typical mobile phase is sodium hydroxide and sodium acetate. When the concentration of the mobile phase is changed, a variety of samples can be separated.
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