Guava TUNEL Assay for detection of late-stage apoptosis
One of the hallmarks of late stage apoptosis, or programmed cell death, is the fragmentation of nuclear chromatin. The DNA degradation generates DNA strands with exposed 3'-hydroxyl ends.
The Guava® TUNEL Assay detects apoptosis-induced DNA fragmentation through a quantitative fluorescence assay. Terminal deoxynucleotidyl transferase (TdT) catalyzes the incorporation of bromo-deoxyuridine (BrdU) residues into the fragmenting nuclear DNA at the 3'-hydroxyl ends by nicked end labeling. A TRITC-conjugated anti-BrdU antibody can then label the 3'-hydroxyl ends for detection by a Guava System. For use with all instruments. a href="http://www.EMD Millipore.com/flowcytometry/fc3/instruments"Click here/a for more information regarding Instrument Compatibility.