Guava TUNEL Assay for detection of late-stage apoptosis One of the hallmarks of late stage apoptosis, or programmed cell death, is the fragmentation of nuclear chromatin. The DNA degradation generates...read more
Guava TUNEL Assay for detection of late-stage apoptosis One of the hallmarks of late stage apoptosis, or programmed cell death, is the fragmentation of nuclear chromatin. The DNA degradation generates DNA strands with exposed 3'-hydroxyl ends. The Guava® TUNEL Assay detects apoptosis-induced DNA fragmentation through a quantitative fluorescence assay. Terminal deoxynucleotidyl transferase (TdT) catalyzes the incorporation of bromo-deoxyuridine (BrdU) residues into the fragmenting nuclear DNA at the 3'-hydroxyl ends by nicked end labeling. A TRITC-conjugated anti-BrdU antibody can then label the 3'-hydroxyl ends for detection by a Guava System. For use with all instruments. a href="http://www.EMD Millipore.com/flowcytometry/fc3/instruments"Click here/a for more information regarding Instrument Compatibility.
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