Guava Mitochodrial Depolorization Kit

Guava Mitochodrial Depolorization Kit
by EMD Millipore, a division of Merck KGaA, Darmstadt, Germany


Guava Mitochondrial Depolarization Assay for detection of early apoptosis Guava Technologies has optimized an assay format for 96-well plates that combines the sensitivity of a single-cell based method with the throughput and ease-of-use of a microplate reader. The Guava® Mitochondrial Depolarization Assay for the Guava EasyCyte™ Plus System allows you to monitor the loss of mitochondrial membran...read more

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Description

 

Guava Mitochondrial Depolarization Assay
for detection of early apoptosis
Guava Technologies has optimized an assay format for 96-well plates that combines the sensitivity of a single-cell based method with the throughput and ease-of-use of a microplate reader. The Guava® Mitochondrial Depolarization Assay for the Guava EasyCyte™ Plus System allows you to monitor the loss of mitochondrial membrane potential through the use of the fluorescent cationic dye, JC-1. An optional dead cell dye, 7-AAD, is also included for discerning apoptotic cells from dead cells.
Distinguishes healthy, early apoptotic, and dead cells
In healthy, non-apoptotic cells, the JC-1 dye enters the mitochondrial matrix, accumulates as aggregates and stains the mitochondria bright red. In apoptotic cells, the mitochondrial membrane potential collapses, and the JC-1 enters the cytoplasm as a monomeric form where it fluoresces green. Results are automatically calculated and presented in a statistical format that is easy to interpret, with total cell counts and percentages for those cell populations that are either healthy, apoptotic, or dead.

Product Overview

Guava Mitochodrial Depolorization Kit by EMD Millipore, a division of Merck KGaA, Darmstadt, Germany
Guava Mitochodrial Depolorization Kit

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