The Guava easyCyte 5HT System provides absolute cell counts and offers other high-demand features, such as five parameters (3 fluorescent colors, 2 light scatter) and has a blue laser for access to commonly used fluorescent dyes. It also offers a built-in 96-well autosampler, which allows researchers to obtain even more data while performing more complex biological studies such as white blood cell phenotyping, cell signaling, cytokine production, activation marker analysis, and multiplex compound screening. Monitoring the interplay of up to five different biological mechanisms simultaneously improves the efficiency of experimental outputs. Not only does this shorten the number of iterations for any one experiment, it also comes that much closer to mimicking the complex biological responses within the body. The Guava easyCyte 5HT System runs all Guava Assays and uses Guava InCyte software for data acquisition and analysis. Guava InCyte offers drag and drop gating, pre and post acquisition compensation, heat map display and IC50/EC50 curve generation.