It is suitable for increased flow rates and is also useful for the purification of large protein complexes. The Strep-tag® / Strep-Tactin® purification system allows the isolation of above 95% pure and functional proteins after one chromatographical step. The Strep-tag system is based on the highly selective and easily controllable interaction between the Strep-tag II peptide and the specially engineered streptavidin called Strep-Tactin.
The binding affinity of Strep-tag II to Strep-Tactin is nearly 100 times higher than to Streptavidin. Gravity flow Strep-Tactin Superflow column, 1 x 1 ml; also available 5 x 0.2 ml , 5 x 1 ml, 1 x 5 ml and 1 x 10 ml columns.
Binding capacity: 50 to 100 nmol recombinant Strep-tag protein (up to 3 mg in case of a 30 KDa protein) per 1 ml bed volume.
Gravity flow Strep-Tactin Superflow column, 1 x 1 ml; also available 5 x 0.2 ml , 5 x 1 ml, 1 x 5 ml and 1 x 10 ml columns.