GPCR dimer assays with Tag-lite® technology

GPCR dimer assays with Tag-lite® technology

Cisbio Bioassays

G-Protein Coupled Receptors (GPCRs) are common drug targets. Previously, most studies were based on the monomeric model, but this perception of GPCRs is now evolving towards homodimeric , hetero...read more

Request Pricing





Receive your quote directly from Cisbio Bioassays for GPCR dimer assays with Tag-lite® technology

Description

 

G-Protein Coupled Receptors (GPCRs) are common drug targets.

Previously, most studies were based on the monomeric model, but this perception of GPCRs is now evolving towards homodimeric , heterodimeric and oligomeric models. To facilitate research into GPCR dimers, CisBio Bioassays has applied the Tag-lite® technology to GPCR interaction assays. Based on HTRF® technology, plasmids have been designed to express either the SNAP-Tag or the CLIP-Tag at the N-terminus of GPCRs, allowing the labelling of two GPCRs (identical or different) with two different fluorescent probes.
Through the TR-FRET signal, interaction between two GPCRs can be investigated.

Applications:

• Receptor deorphanization: a GPCR could be deorphanized by a drug through the characterization of a GPCR heterodimeric complex.
• New GPCR targets: to identify heterodimer specific drugs or bivalent drugs on homodimers.

Tag-lite plasmids are available for many targets:

Adenosine A1, A3
Adrenergic Alpha1A, Beta2 
Anaphylatoxin C5a
Angiotensin AT1, AT2
Bombesin BB2, BRS3
Bradykinin B1, B2
Cannabinoid CB1
Chemokine CCR1, CCR2, CCR3, CCR4, CCR5, CCR6, CX3CR1, CXCR1, CXCR2, CXCR3, CXCR4, CXCR5, CXCR6, CXCR7 
Cholecystokinin CCK1, CCK2
Corticotropin-Releasing Factor CRF1
Dopamine D1, D2, D2L
Endothelin ETA, ETB
Galanin GAL2
Ghrelin GHSR1, A 
Glucagon GIPR, GLP1, GLP2
Growth-releasing hormone GHRHR
Histamine H1, H2 
Leukotriene/lipoxin CysLT1, CysLT2
Lysophosphatidic acid receptor EDG4
Melanocortin MC2, MC3, MC4, MC5
Metabotropic glutamate mGluR5
Muscarinic M3
Neuropeptide FF NPFFR1    
Neuropeptide Y Y1, Y2, Y5
Neurotensin NTS1
N-formylpeptide FPR2
Opioid Delta opioid, Kappa opioid, Mu opioid 
Orexin OX1, OX2 
Parathyroid PTH1 
Prolactin-releasing peptide PRRP 
Serotonin 5HT1A, 5HT2A, 5HT2B, 5HT2C
Tachykinin NK1, NK2 
Vasoactive Intestinal Peptide PAC1, VPAC1, VPAC2 
Vasopressin/Oxytocin V1a, V2 

Please contact CisBio for more information on other GPCRs.

Feedback

Request info

To:Cisbio Bioassays
From:
Please contact me / call me with the GPCR dimer assays with Tag-lite® technology,

I am looking to purchase in /

.
About you

Write a review

Sharing your experience will help scientists like you. Achieve Reviewer Status and Win an iPad air (All reviews published will be entered into the next drawing on May 30th 2014).

  
Your Email

 



Ease of use

After sales services

Value for money

WATCH:
The benefits of becoming a reviewer
Share your opinions...()
HINTS & TIPS
Give reasons for the star ratings you have awarded for each area.
Would you recommend this to a colleague?
What would you like to have known about equipment and consumables before you purchased?



Why make an enquiry with SelectScience?

  • EASY It’s quick and simple to do
  • FAST Your enquiry will be delivered straight to the manufacturer
  • FREE You’re under no obligation
  • SECURE We only pass your details on to trusted suppliers at your request
  • SAVE TIME Submit your details once and make multiple enquiries
SelectScience Trusted Banner