GoTaq® 2-Step RT-qPCR System

 5.0/5.0 (1 review)

Promega Corp.

GoTaq®2-Step RT-qPCR System is a reagent system for quantitative analysis of RNA using a two-step reverse transcription-quantitative PCR (RT-qPCR) method. The first step consists of cDNA synthesis...read more

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Reviews

Daniel Filho


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Member since 2006

Organization:
Universidade Federal do Acre
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RATING: 5.0

  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 15 Apr 2014

Application Area: Cancer Research
GoTaq® 2-Step RT-qPCR System

"GoTaq® 2-Step RT-qPCR System is a kind of product I recommend to any PCR routine laboratory; It made my work easy and I had good results using it. "

Description

 

GoTaq®2-Step RT-qPCR System is a reagent system for quantitative analysis of RNA using a two-step reverse transcription-quantitative PCR (RT-qPCR) method.

The first step consists of cDNA synthesis using the GoScript™ Reverse Transcription System. Optimized reagents and ultra-active, GoScript™ Reverse Transcriptase result in high-efficiency, full-length cDNA synthesis from rare and abundant targets with up to 5 µg of RNA input. In the second step, cDNA is quantified using the GoTaq®qPCR Master Mix.

Formulated with a hot-start DNA polymerase, the GoTaq® qPCR Master Mix is optimized for reproducibly specific, sensitive, highly efficient qPCR using the standard or fast mode of a real-time PCR instrument. The GoTaq® qPCR Master Mix contains BRYT™ GREEN dye which can be twice as bright as of SYBR® Green I. The superior performance of these two steps allows researchers to observe earlier quantification cycle values over broader linear ranges.

The GoTaq® 2-Step RT-qPCR System provides a ready-to-use kit for analyzing a wide range of RNA targets by combining the high-activity of GoScript™ Reverse Transcription System with the ultra-bright fluorescence of GoTaq® qPCR Master Mix. The system is robust, requires little optimization, is flexible for a variety of applications, and carries the PCR performance guarantee.

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