Goat TrueBlot® Western Blot Kit 50 uL by eBioscience

The Goat TrueBlot® Western Blot Kit contains the critical supporting reagents, buffers, and substrates for immunoprecipitation and Western blotting of samples using TrueBlot second step immunoblotting reagents in conjunction with your own primary IP antibody and primary (Goat IgG) Western blotting antibody. TrueBlot technology enables unhindered detection of protein bands of interest which would otherwise be obscured by the presence of reduced denatured heavy and light chain immunoglobulin in the blot (as detected by the conventional immunoblotting HRP anti-Goat IgG reagent). Goat IgG TrueBlot is the unique horseradish peroxidase conjugated anti-Goat IgG immunoblotting second step reagent which enables detection of immunoblotted target protein bands, without hindrance by interfering immunoglobulin heavy and light chains from your IP antibody. Use it in place of your usual HRP anti-Goat IgG immunoblotting second step reagent. It is easy to generate publication-quality IP/WB data with Goat IgG TrueBlot. Note that there are two key procedural considerations: 1. Immunoprecipitate should be completely reduced. 2. Milk should be used as the blocking protein for the immunoblot. The Goat TrueBlot Western Blot Kit components are sufficient for 20-25 miniblots.