Status:
Advanced ReviewerMember since: 2015
Organization: University of Manchester
Great for GFP pulldown
Application Area: Pulldowns for analysis by western blot or proteomics
"Really simple to use, very efficient. We use magnetic bead product for great results, better than standard IP and less worry of usual heavy and light chain IgG contamination/masking."
Status:
Advanced ReviewerMember since: 2015
Organization: University of Manchester
Application Area:Pull down GFP and GFP tagged proteins from cell lysates
"This is an excellent product. Both the agarose and magnetic bead formats are extremely efficient for immunoprecipitation of GFP tagged proteins, and the usual heavy and light antibody chain contamination is avoided."
GFP -Trap® - use your fluorescence fusion as tag for biochemical studies.
Curiously GFP, the most widely used labeling tag in cell biology, is rarely used for biochemical analyses. This may be due in part to limited GFP antibody availability and specificity as well as co-eluted heavy and light antibody chains that interfere with subsequent analyses.
Our GFP-Trap® is a high quality GFP-binding protein coupled to a monovalent matrix (agarose beads, magnetic particles or in 96-multiwell plates) for biochemical analysis of GFP fusion proteins and their interacting partners.
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