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Member since: 2012
Organization: Wayne State University
This instrument streamlined and standardized our tumor processing and is bringing us fantastic results compared to our method before we purchased the GentleMACS Dissociator.
Application Area: Preparation of single cell suspensions of tumor cells from freshly dissected patient tumor samples
"The GentleMACS Dissociator and accompanying enzyme kits have dramatically improved our yield of viable patient-derived tumor cell lines and mouse xenograft tumor cell lines. Before we began using the GentleMACS our success rate at generating cell lines was about 50% from glioblastomas and 25% from brain metastases. Now with the GentleMACS we are over 85% successful with both. Following the supplied protocol is very easy, and not terribly time-consuming, which is important when working with patient specimens. Now we dream of getting the funds to upgrade our lab from the 2 sample model up to the Octo-Dissociator which can run 8 at once, as implied by the name :) "
Member since: 2014
Organization: Universidad de Extremadura
Application Area:Tissue disgregator for cell and nucleic acids isolation
"The system allows to work up to 8 different samples simultaneously. Mechanical dissociation can be applied in varied ways (speed, time and rotation sense ). Temperature (37ºC) can be used at the same time, allowing a combination of enzymatic and mechanical strategies. All of this, produces an increase in cell viability and cell recovery and permits to reduce the size of starting material. We use it for RNA and DNA extraction, obtaining amazing results. Price is the only drawback. "
Member since: 2013
"This apparatus has multiple programs to fit with different biological applications and it is possible to upgrade it or create its own program. It is reproducible and very convenient to use. We still need to buy specific tubes developed by Miltenyi to use it. "
Member since: 2009
Organization: University of Minnesota
Application Area:Tissue dissociation
"Easy to use. The single cell suspensions which are created allow for much more robust cell line generation."
Member since: 2011
Organization: Imperial College
"Product is very easy to use and customer support when ordering/installing/after care was great. We developed protocol for tissue dissociation at our site together with sales manager from Miltenyi. I hope the price will decrease when more people start buying this machine as at the moment it is a bit pricy, though it is a one time instrument."
Two types of unique gentleMACS Tubes used with the instrument enable the time-saving and easy dissociation or homogenization of tissues in a closed system. C Tubes are used for the gentle preparation of single-cell suspensions from tissues, whereas M Tubes are used for the homogenization of tissues or cells. A single sample or two samples in parallel can be processed. The instrument offers optimized gentleMACS Programs for a variety of specific applications. The standardized tissue dissociation or homogenization procedures make for reliable and reproducible results.
The gentleMACS Dissociator:
• Time-saving automated tissue dissociation
• Standardized procedure
• High level of user safety
• Sterile sample handling