GenElute™ HP 96-Well Plasmid Miniprep Kit by Merck

The GenElute™ HP Plasmid Miniprep Kit offers a fast and efficient solution for high-throughput plasmid preparation from E. coli cultures. This kit combines silica-based membrane technology with the convenience of vacuum manifold or automated liquid handling workstation format. Following lysis the DNA is bound to the silica membrane and contaminants are removed with two simple wash steps. The DNA is then eluted in water or TE buffer by centrifugation. The recovered plasmid DNA is ready for immediate use in applications such as restriction enzyme digestion, sequencing, PCR, and transfections.

• From harvested bacterial culture to pure plasmid DNA in under 50 minutes
• Up to 10 µg yield of high-copy plasmid DNA per well
• Optimized for best-in-class production of virus titers from Mission shRNA plasmid vectors
• Generates plasmid DNA for a wide variety of downstream applications, including transfections, sequencing, and restriction digests
• Offers the flexibility of both manual and automated formats
• No phenol/chloroform extraction or alcohol precipitation required
• Kits are stable at room temperature for convenient storage

An overnight recombinant E. coli culture is harvested with centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the plasmid DNA onto silica in the presence of high salts. Vacuum wash steps then remove contaminants. Finally, the bound plasmid DNA is eluted in water or Tris-EDTA buffer.