GENEius - The Tuning Tool by Eurofins Genomics

Optimise your sequences for gene synthesis.

Gene optimisation is an important factor for successful protein expression. Eurofins Genomics has developed a proprietary
software tool for gene optimisation, called GENEius.

GENEius optimises all protein coding sequences from short DNA fragments of 100 bases to long genes with >12,000 bases. It adapts either DNA or amino acid sequences.

GENEius uses codon usage tables of the heterologous expression host in order to improve protein expression:

• Usage of codons with high frequencies based on the selected organism
• Avoidance of rare codons of the expressing organism
• Removal of unwanted motifs or secondary structures like repeat or hairpin structures
• Evenly distributes G and C bases