GeneArt® Seamless PLUS Cloning and Assembly Kit by Thermo Fisher Scientific Invitrogen
Manufacturer Thermo Fisher Scientific Invitrogen
For highly-efficient, simultaneous, and seamless in vitro assembly of up to 4 DNA inserts plus any vector totaling up to 40 kb in length in a pre-determined order.
GeneArt® Seamless PLUS Cloning is a simple, two-step process, consisting of in vitro assembly followed by transformation into One Shot® DH10B™ T1R SA competent E. coli. The kit employs a proprietary enzyme/buffer mix to assemble DNA fragments with shared terminal end homology without extra sequences or scars in the final construct ("... Read more
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GeneArt® Seamless PLUS Cloning and Assembly Kit
Average Rating: 4.7
1 Scientist has reviewed this product
Status:
Reviewer
Member since: 2009
Organization: University of Milan Bicocca
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Rating: 4.7
Application Area: Cancer Biology
"It’s simple and convenient to use, with highly efficient cloning of large single fragments or multiple small fragments. The efficiency of reproducibility is high for multiple insert cloning compared to other cloning kits. Because of its ease and efficiency it is used for multiple cloning. The accuracy and sensitivity were achieved predominantly with each cloning. My personal experience with no additional errors suggests that this product is best used for cloning up to 25kb. "
Review date: 06 Aug 2014 | GeneArt® Seamless PLUS Cloning and Assembly Kit
For highly-efficient, simultaneous, and seamless in vitro assembly of up to 4 DNA inserts plus any vector totaling up to 40 kb in length in a pre-determined order.
GeneArt® Seamless PLUS Cloning is a simple, two-step process, consisting of in vitro assembly followed by transformation into One Shot® DH10B™ T1R SA competent E. coli. The kit employs a proprietary enzyme/buffer mix to assemble DNA fragments with shared terminal end homology without extra sequences or scars in the final construct ("seamless"). Terminal end homology is easily incorporated by PCR amplification with custom DNA oligos engineered using our free web tool.
With the GeneArt® Seamless PLUS Cloning and Assembly Kit, the main factors effecting cloning efficiency are the size of the DNA fragments (100 bp to 10 Kb), the total size of the final molecule (≤ 40 Kb), and the quality and specificity of each fragment.
Cloning success is independent of the insert sequence and vector type, allowing you to design and add nearly any desired sequence, or combination of sequences, to any plasmid as long as it can be linearized by either restriction enzyme digestion or PCR. The circularized clones obtained from the reaction contain only the sequence of your original vector, inserts, and designated homologies, with no extraneous nucleotide insertions.
Advantages:
GeneArt® Seamless PLUS Cloning is a simple, two-step process, consisting of in vitro assembly followed by transformation into One Shot® DH10B™ T1R SA competent E. coli. The kit employs a proprietary enzyme/buffer mix to assemble DNA fragments with shared terminal end homology without extra sequences or scars in the final construct ("seamless"). Terminal end homology is easily incorporated by PCR amplification with custom DNA oligos engineered using our free web tool.
With the GeneArt® Seamless PLUS Cloning and Assembly Kit, the main factors effecting cloning efficiency are the size of the DNA fragments (100 bp to 10 Kb), the total size of the final molecule (≤ 40 Kb), and the quality and specificity of each fragment.
Cloning success is independent of the insert sequence and vector type, allowing you to design and add nearly any desired sequence, or combination of sequences, to any plasmid as long as it can be linearized by either restriction enzyme digestion or PCR. The circularized clones obtained from the reaction contain only the sequence of your original vector, inserts, and designated homologies, with no extraneous nucleotide insertions.
Advantages:
- Increased Efficiency: pre-cloning option for large fragments for increased cloning efficiency
- Larger Constructs: create constructs up to 40 kb
- Versatility: high capacity, broad-range conjugative vector that replicates in most Gram negative bacteria
Benefits:
- Speed and Ease—clone up to 4 DNA fragments, with sequence of your choice, simultaneously in a single vector; no restriction digestion, ligation, or recombination sites required
- Precision and Efficiency—designed to let you clone what you want, where you want, in the orientation you want, and achieve up to 90% correct clones with no extra sequences left behind
- Free Tools—design your final construct and DNA oligos in silico using our free web-based tool that takes you step-by-step through your project
- Vector Flexibility—use our linear vector or a vector of your choice
- Diverse Applications—streamline many synthetic biology and molecular biology techniques through the rapid combination, addition, deletion, or exchange of DNA segments
Product Overview
GeneArt® Seamless PLUS Cloning and Assembly Kit
Manufacturer Thermo Fisher Scientific Invitrogen
4.7 / 5.0 | 1 reviews