All-in-one vector system for CRISPR-based genome editing.
Transfect, enrich, screen, and publish—using our GeneArt® CRISPR Nuclease Vector Kit. The clustered regularly interspaced short palindromic repeats (CRISPR) Nuclease system offers a simple, ready-to-use, all-in-one expression vector system consisting of both a Cas9 nuclease expression cassette and a guide RNA cloning cassette for rapid and efficient cloning of a target-specific crRNA. This system allows you to edit and engineer the genomic locus of your choice in a sequence-specific manner from a single plasmid. After relevant targets have been identified with GeneArt® CRISPRs, the biologically-relevant mutations can be validated with GeneArt® Precision TALs to reduce potential off-targeting.
GeneArt® CRISPR Nuclease Vector Kits are reporter vector systems for expression of the functional components needed for CRISPR-Cas genome editing. The kits facilitate the generation of constructs to express noncoding guide RNA (including crRNA and tracrRNA), using a plasmid vector that also expresses Cas9 endonuclease.