GeneArt® CRISPR Nuclease mRNA by Thermo Fisher Scientific Invitrogen

The GeneArt® CRISPR Nuclease mRNA is wild type Cas9 as mRNA, for genome editing using CRISPR-Cas9 technology. Ready-to-transfect Cas9 mRNA circumvents the need for time-consuming cloning steps required when using CRISPR vector systems. Cas9 mRNA can be co-transfected with in vitro transcribed guide RNA (IVT gRNA) or a synthetic gRNA expression cassette containing U6 promoter. gRNA expressing cassette and IVT gRNA template can be ordered as GeneArt® CRISPR Strings™ DNA, a 500bp DNA fragment. Following transfection, the Cas9 protein is directed by gRNA to target specific genomic locus.

This system allows multiplex genome editing, where multiple target gene sequences can be edited simultaneously in a single transfection reaction with the addition of multiple gRNAs. The system is versatile and simple to use, and changing target specificity only requires a change in the design of the GeneArt® CRISPR Strings™ DNA.