G-LISA™ Small G-protein Activation Assays by Cytoskeleton Inc.
Manufacturer Cytoskeleton Inc.
The G-LISA™ assay uses a 96-well plate coated with RBD domain of Rho-family effector proteins. The active GTP-bound form of the Rho-family protein, but not the inactive GDP-bound form, from a biological sample will bind to the plate. Bound active Rho-family protein is then detected by incubation with a specific primary antibody followed by a secondary antibody conjugated to HRP. The signal is then developed with OPD or chemiluminescence reagents. These assays are simple, fast (<3 hours), requ... Read more
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G-LISA™ Small G-protein Activation Assays
Average Rating: 4.3
1 Scientist has reviewed this product
Status:
Reviewer
Member since: 2010
Organization: NORTHWESTERN UNIV. SCHOOL OF MEDICINE
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- Ease of use
- After sales service
- Value for money
Rating: 4.3
Application Area: Signaling research
"Excellent kit, works every time. But it's very expensive, so I would recommend using it only when it's important to get results fast."
Review date: 07 Jun 2012 | G-LISA™ Small G-protein Activation Assays
The G-LISA™ assay uses a 96-well plate coated with RBD domain of Rho-family effector proteins. The active GTP-bound form of the Rho-family protein, but not the inactive GDP-bound form, from a biological sample will bind to the plate. Bound active Rho-family protein is then detected by incubation with a specific primary antibody followed by a secondary antibody conjugated to HRP. The signal is then developed with OPD or chemiluminescence reagents. These assays are simple, fast (<3 hours), require only small amounts of sample (5-25 µg cell protein), yield quantitative and accurate data and are suitable for high thoughput screens.
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