FuGENE® HD Transfection Reagent

FuGENE® HD Transfection Reagent
 4.2/5.0 (6 reviews)

Roche Applied Science - a member of the Roche Group

Roche FuGENE HD Transfection Reagent is a next-generation transfection reagent, free of animal-derived components. Roche FuGENE HD Transfection Reagent is designed to transfect a wide range of eu...read more

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Reviews

Arne Meyer


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Member since 2013

Organization:
University of Basel
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RATING: 4.3

  • 5 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 03 Jan 2013

Application Area: Toxicology
FuGENE® HD Transfection Reagent

"This is a very good transfection agent that provides high efficacy. Also, it has low toxicity and is good value."

Read 6 reviews

Arun Kumar Nalla


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Member since 2011

Organization:
UNIV. OF ILLINOIS COLLEGE OF MEDICINE AT PEORIA
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RATING: 4.0

  • 4 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 10 Aug 2011

FuGENE® HD Transfection Reagent

"Transfection efficiency is around 70%. it works fine both in over expression and shrna plasmid on a variety of cell lines."

JACQUELINE JOHNSON


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Member since 2011

Organization:
H. LEE MOFFITT CANCER CTR.
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RATING: 5.0

  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 05 Jul 2011

FuGENE® HD Transfection Reagent

"Awesome transfection reagent for the cost."

Sajani Lakka


Status:


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Member since 2009

Organization:
UNIV. OF ILLINOIS COLLEGE OF MEDICINE
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RATING: 4.0

  • 4 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 11 May 2009

FuGENE® HD Transfection Reagent

"Very good to transfect medulloblastone cells."

Byoung Dae Lee


Status:


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Member since 2007

Organization:
Johns Hopkins Univ
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RATING: 4.0

  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 2 out of 5VALUE FOR MONEY




Review date: 11 Jan 2008

FuGENE® HD Transfection Reagent

S. Lin


Status:


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Member since 2007

Organization:
University of Maryland
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RATING: 4.0

  • 5 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 3 out of 5VALUE FOR MONEY




Review date: 08 Jun 2007

FuGENE® HD Transfection Reagent

"Excellent transfection rate."

Description

 

Roche FuGENE HD Transfection Reagent is a next-generation transfection reagent, free of animal-derived components.

Roche FuGENE HD Transfection Reagent is designed to transfect a wide range of eukaryotic cells, including insect cells and many tumor cell lines not transfected well by other reagents (e.g., MCF7, Hep G2, PC-3, HeLa, MA-10, Hep G2, SH-SY5Y, A7r5, STO, SCC-61, SQ20B, T98G, STSAR-90, A375, A549, and others). In addition, embryonic stem cells are effectively transfected with FuGENE® HD Transfection Reagent.

Roche FuGENE HD Transfection Reagent can be successfully used in a variety of applications, such as gene expression analysis and protein production using transiently transfected cells, generation of stable cell lines, generation of retroviruses and lentiviruses, expression of shRNA for Gene Knockdown studies, in drug discovery programs, and for target validation.

Features of Roche FuGENE® HD Transfection Reagent:

  • Achieve new levels of transfection efficiency in difficult-to-transfect cell lines, such as RAW 264.7 and many tumor cell lines that are not transfected well by other reagents.
  • Select FuGENE® HD Transfection Reagent for the transfection of stem cells and obtain superior results, as demonstrated in a publication (Biochemica, 4, 2006; 24 – 26) where the reagent was compared with a pool of 37 other transfection reagents.
  • Generate physiologically relevant data you can trust by using a reagent that has exceptionally low cytotoxicity, as measured through the amount of off-target effects and cell proliferation.
  • Obtain higher expression levels of proteins in many adherent and suspension-adapted eukaryotic cell lines (HEK-293, CHO-S, CHO-K1, COS-7) and insect cell lines such as High Five and Sf9, over extended periods with scalability that other reagents cannot provide.
  • Accelerate the move to development by using this unique non-liposomal reagent that is free of animal-derived components, stable at room temperature, sterile (0.1 µm filtered), and active in up to 100% serum. The ability to transfect cells in up to 100% serum provides a good model to mimic biological conditions.
  • Increase experimental throughput and enable target validation by using a stable reagent with a simple, consistent, and fast protocol across a wide range of cell types.

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