Freedom EVO® Protein Purification
Tecan has teamed up with GE Healthcare to provide an automated solution for the screening of best producing clones on Freedom EVO® using GE Healthcares MultiTrap® plates. The MultiTrap plates are filled with Chromatography Media in a 96 well format for His- / GST tagged proteins, further formats include Protein A, Streptavidin, and Desalting.
The new MultiTrap™ prepacked 96-well filter plates from GE Healthcare are designed and fully tested for screening of recombinant proteins on Freedom EVO®. The combination of Tecan's robotic integration and liquid handling expertise together with the versatile MultiTrap platform gives the user a fully automated protein purification system for higher throughput demands.
Proteins can be extracted from E. coli cultures without centrifugation or filtration, leading to a more efficient workflow. Purification can be carried out either under vacuum pressure or by centrifugation.
The system addresses the processing of both His-tagged as well as GST-tagged fusion proteins in 96-well plate format, with low cross-contamination, high reproducibility, and highly robust processing. MultiTrap plates are also available for screening of antibodies, parallel enrichment of the target protein with immuno-affinity technique and desalting/buffer exchange. Purification can be easily scaled up on the ÄKTAxpress™ system from GE Healthcare.