The Cell Seed and Feed Workstation implements a number of features to help maintain sterile conditions. All pipetting of samples occurs on the deck of the Freedom EVO which is enclosed in a positive pressure HEPA filtered environment. UV lights integrated into the enclosure can be used when the system is not running. Additionally as routine maintenance during idle time, the liquid handling paths are filled with an ethanol solution. The result is a system that can maintain sterility for extended periods of time.