The FP.3 accessory employs Chirascan’s photoelastic modulator (PEM) and a single photomultiplier detector for fluorescence polarisation/anisotropy measurements. This configuration provides greater sensitivity and a simpler instrument configuration than conventional fluorescence polarisation instrumentation. Conventional fluorescence polarisation measurements are made using a T-format design featuring two detectors and three polarisers; one for generating the polarised excitation light and two for collection of the horizontal and vertical components of the fluorescence signal from which the fluorescence polarisation (or anisotropy) value is derived. The sensitivity of this technique is limited by the use of the emission polarisers and differences in sensitivity between the fluorescence det...Read more
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