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Member since: 2014
Organization: University of Liverpool
Application Area: Genomics
"Fosmid library prepared from cow lymphocytes sample. Samples collected in Africa and lymphocytes prepared using Lymphoprep after storage at ambient temperature (23-35C) for 24-48 hours, so less than ideal conditions and dead cells may have been present. LGC prepared DNA from several samples before obtaining DNA of adequate quality for Fosmid library prep. Finally supplied high titre fosmid library split into 192 pools together with DNA for each pool that we used for genome sequencing without further purification. I was impressed by the effort put into dealing with some tricky samples and good communication about progress and issues as they arose."